K-562-CAS9 cell line

location： Home > Products > Cas9 Stable Cell Lines > K-562-CAS9

All products

K-562-CAS9 Order

Catalog#

YC-C023-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture Medium-I

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

K-562-CAS9

Morphology

Lymphocyte-like,suspension

Passage ratio

1：3-1：5

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%IMDM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the K-562 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a K-562-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
K-562-CAS9	Cas9	18.70168877	2.00867081
K-562-CAS9	h-actin-B	16.69301796	2.00867081
K-562	Cas9	34.68590164	17.27577781
K-562	h-actin-B	17.41012383	17.27577781

Cutting Efficiency Validation

K-562-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the K-562-CAS9 stable cell pool which is electroporated by CHD4 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:K-562			STR Info (Cell bank) Cell Line:K-562
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	8	8		8	8
D7S820	9	11		9	11
D16S539	11	12		11	12
VWA	16	16		16	16
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	9	10		9	10

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

Related Popular Products

KO Cell Lines

RBKS Knockout cell line(K-562)
inquiry More details

Organism: Human

Gene Name: RBKS

Gene ID: 64080

Size: 1*10^6

Catalog#: YKO-H303

YKO-H303(K-562)
RAPSN Knockout cell line(K-562)
inquiry More details

Organism: Human

Gene Name: RAPSN

Gene ID: 5913

Size: 1*10^6

Catalog#: YKO-HJ102

YKO-HJ102(K-562)
SET Knockout cell line(K-562)
inquiry More details

Organism: Human

Gene Name: SET

Gene ID: 6418

Size: 1*10^6

Catalog#: YKO-HJ101

YKO-HJ101(K-562)

Luciferase Stable Cell Lines

K-562-Luc
More details Order

Catalog#: YC-C023-Luc-P

Size: 1*10^6

Instruction:

Wild-type Cell Lines

Human Chronic Myelogenous Leukemia Cell Line(K-562)
More details Order

Catalog#: YC-C023

Size: 1*10^6

Instruction:

Related Popular Products

KO Cell Lines

RBKS Knockout cell line(K-562)

Organism: Human

Gene Name: RBKS

Gene ID: 64080

Size: 1*10^6

Catalog#: YKO-H303

inquiry More details
RAPSN Knockout cell line(K-562)

Organism: Human

Gene Name: RAPSN

Gene ID: 5913

Size: 1*10^6

Catalog#: YKO-HJ102

inquiry More details
SET Knockout cell line(K-562)

Organism: Human

Gene Name: SET

Gene ID: 6418

Size: 1*10^6

Catalog#: YKO-HJ101

inquiry More details

Luciferase Stable Cell Lines

K-562-Luc

Price (USD): -

Catalog#: YC-C023-Luc-P

Size: 1*10^6

Instruction:

inquiry More details

Wild-type Cell Lines

Human Chronic Myelogenous Leukemia Cell Line(K-562)

Price (USD): -

Catalog#: YC-C023

Size: 1*10^6

Instruction:

inquiry More details

✖

Phone

✖

Contact us✖

* Name：

Institution：

* Project Description：

* How would you like us to contact you?

* E-mail：

Phone：

WhatsAPP：

FaceBook Messenger：

Our Solution Specialists will contact you within 2 business days. Of course, you can also tell us when you would like to be contacted:

Select：

K-562-CAS9 cell line

Subscribe Us

K-562-CAS9 Order

Detailed Product Information

Product Advantage

Related Popular Products

KO Cell Lines

RBKS Knockout cell line(K-562) inquiry More details

RAPSN Knockout cell line(K-562) inquiry More details

SET Knockout cell line(K-562) inquiry More details

Luciferase Stable Cell Lines

K-562-Luc More details Order

Wild-type Cell Lines

Human Chronic Myelogenous Leukemia Cell Line(K-562) More details Order

Related Popular Products

KO Cell Lines

RBKS Knockout cell line(K-562)

RAPSN Knockout cell line(K-562)

SET Knockout cell line(K-562)

Luciferase Stable Cell Lines

K-562-Luc

Wild-type Cell Lines

Human Chronic Myelogenous Leukemia Cell Line(K-562)

Gene Editing Services

EZ-editor™ Products

Contact us✖

RBKS Knockout cell line(K-562)
inquiry More details

RAPSN Knockout cell line(K-562)
inquiry More details

SET Knockout cell line(K-562)
inquiry More details

K-562-Luc
More details Order

Human Chronic Myelogenous Leukemia Cell Line(K-562)
More details Order